Modeling lymphangiogenesis in a three-dimensional culture system

Modeling lymphangiogenesis in a 3D-culture system

Unraveling the molecular mechanisms of lymphangiogenesis is hampered by the lack of appropriate in vitro models of three-dimensional (3D) lymph vessel growth which can be used to exploit the potential of available transgenic mice. We developed a potent reproducible and quantifiable 3D-culture system of lymphatic endothelial cells, the lymphatic ring assay, bridging the gap between 2D-in vitro a...

Modeling human tumor angiogenesis in a three-dimensional culture system.

The intrinsic complexity of the process of vessel formation limits the efficacy of cellular assays for elucidation of its molecular and pharmacologic mechanisms. We developed an ex vivo three-dimensional (3D) assay of sprouting angiogenesis with arterial explants from human umbilical cords. In this assay, human arterial rings were embedded in basement membrane extract gel, leading to a network ...

P105: Inhibition of Vasculogenic Mimicry in a Three-Dimensional Culture in Glioblastoma

Glioblastoma is one of the most common primary brain tumors (80% of patients) that has a poor prognosis due to malignancy. Glioblastoma has an annual incidence of 5.26 per 100 000 population or 17 000 new diagnoses per year and so as the population aging, the number of patients is expected to increase. There is a growing body of literature investigating the tumor microenvironmenta...

three-dimensional modeling and simulation of ohmic heating of processing in a two-phase food system

the basis of the ohmic heating process is the transmission of alternating electric current through multi-phase solutions that is followed by heat generation due to particle resistance to the transmitted electric current. throughout the present study, simultaneous transfer of heat and electricity was modeled in a two-phase system of solid-liquid food to investigate the critical factors affecting...

Low Doses of Celecoxib Stimulate Human Endometrium Growth in A Three-Dimensional Culture Model